Standardized, Scalable, and Timely Flexible Adeno-Associated Virus Vector Production Using Frozen High-Density HEK-293 Cell Stocks and CELLdiscs
نویسندگان
چکیده
منابع مشابه
High-titer, wild-type free recombinant adeno-associated virus vector production using intron-containing helper plasmids.
Recombinant adeno-associated virus (rAAV) is capable of directing long-term, high-level transgene expression without destructive cell-mediated immune responses. However, traditional packaging methods for rAAV vectors are generally inefficient and contaminated with replication-competent AAV (rcAAV) particles. Although wild-type AAV is not associated with any known human diseases, contaminating r...
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To further understand the biology of adeno-associated virus (AAV) and identify the presence of AAV in laboratory samples, we have developed a sensitive PCR-based assay using degenerate primers based on the sequence of seven diverse AAV isolates. Using these primers, we can detect free virus in viral stocks, cleared cell lysate, as well as in latently infected cells. The method can detect as lit...
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To develop new recombinant monoclonal antibody fragments for therapy and imaging, it is indispensable to have a simple and easy procedure to handle the eukaryotic expression system for production of proteins in high amounts. Gene amplification techniques such as the dehydrofolate reductase (DHFR) system in Chinese hamster ovary cells or the glutamine synthase system in myeloma cells have a coup...
متن کاملThe ethics of HEK 293.
1 Congregation for the Doctrine of the Faith, Donum vitae (February 2, 1987), Introduction, 5. 2 Ibid., I, 1. The topic of embryonic stem cells is very much in the forefront of public interest. The moral stance on obtaining stem cells from embryos is clear: The Church has always taught that all human life from the very beginning must be respected and protected. If human embryos are killed to ob...
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A variety of gene transfer strategies have been developed to treat inherited, degenerative, and acquired diseases. Among the different vector systems developed so far, recombinant adeno-associated viral (AAV) vectors have shown notable benefits, including prolonged gene expression, transduction of both dividing and nondividing cells, and a lack of pathogenicity caused by wild-type infections. T...
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ژورنال
عنوان ژورنال: Human Gene Therapy Methods
سال: 2019
ISSN: 1946-6536,1946-6544
DOI: 10.1089/hgtb.2018.228